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Scientist I/II, Cell and Molecular Biologist

Company: AbbVie
Location: Crystal Lake
Posted on: January 10, 2020

Job Description:

The Genomics Research Center (GRC) is an AbbVie center of excellence for genetics and genomics research to support both Discovery and Development pipelines. The GRC is composed of five core functional areas including Genomic Technologies, Bioinformatics, Pharmacogenomics, Human Genetics and Functional Genomics, which collectively play an integral role towards the goal of finding the right targets, understanding human disease biology, and investigating patient responses to our drugs in clinical trials. The Department of Functional Genomics within the GRC provides expertise in genome editing and CRISPR technology to discover and validate novel drug targets for Immunology, Oncology and Neuroscience discovery pipelines. We utilize cutting edge genomic techniques to transform human genetics data into drug targets and to identify pathogenetic mechanisms. We apply high-throughput functional genomic screening approaches to in vitro and in vivo preclinical models with diverse molecular and phenotypic readouts. We perform hypothesis-driven research for target validation and execute mechanistic studies utilizing a variety of genomic and non-genomic tools in primary and in vitro cell culture systems. We have an exciting opportunity for a Scientist to join our collaborative team of geneticists, molecular and cellular biologists, and computational biologists. Key Responsibilities Include: Perform CRISPR/Cas9 functional genomics research based on lentiviral and RNP delivery methods Participate in cross-functional target identification and validation projects to support early drug discovery efforts in oncology, immunology, immuno-oncology, and neuroscience Develop cell-based functional assays using cell lines and primary cells Perform arrayed (96 and 384-well plate) and pool CRISPR screens, using fluorescent cell sorting or high content imaging assays, and analyze screening data using various bioinformatic and statistical software Operate the CompacT SelecT automated cell culture system for cell line maintenance and expansion Collaborate extensively with scientists across diverse functional and therapeutic areas in matrix research environment Position will be hired based on level of experience. Bachelors or Masters in cell and molecular biology, or in a related discipline, with typically 2-5+ (MS) or 5-7+(BS) years of experience in laboratory research Extensive experience in cell-based functional assay development is required. Experience in transfection, nucleofection, lentiviral transduction, flow cytometry, or iPSC, and primary cells is desired but not required. Experience in HTS lab automation, liquid handling, or high-content cell imaging analysis is required. Strong skills in molecular analytic methods including DNA/RNA isolation, PCR primer design, molecular cloning, genotyping, qPCR, and western blot will be favorably considered. Basic conceptual understanding of broad genomic technologies such as next-gen sequencing, RNA Seq, RNAi (shRNA/siRNA), and CRISPR gene editing is required. Ability to learn new skills and apply them in a fast-paced research environment is a must. Demonstrated creativity, problem-solving skills, and attention to details are required. Strong communication and collaboration skills are desired. Ability to work independently as well as in a team is a must.

Keywords: AbbVie, Crystal Lake , Scientist I/II, Cell and Molecular Biologist, Other , Crystal Lake, Illinois

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